Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKSFILSTGSYLPKKKLGNDEIALMVETSDEWIRQRTGITQRYIADEAELTSDLAVNSAKNAIEKAQISVDEIGLIIVATTTPDKTLPSCATIAQNKLKCKNAFSFDVQAACSGFIYAVTIADSLIKSNDRIKYALVIGAEIMSRIVDWKDRSTCVLFGDGAGAVIMKSTAHCNEMTENSTRGIISTNLYSDGNV-DVLCTKGGISSTGD-SGKIYMNGREVFKHAVDKLTASIEETLRCNNLKITDIDWLVPHQANIRIIEAVVKKLNFLMEKVINTVDQHANTSAASIPLALDYAIQKPKIKPGSLGILVAIGAGLTWGSVLLRY 1HNJ Chain:A ((1-317)) -MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHIAAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKGCPAFDVAAACAGFTYALSVADQYVKSG-AVKYALVVGSDVLARTCDPTDRGTIIIFGDGAGAAVLAASEE---------PGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVTELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTSAASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF

General information: TITO was launched using: RESULT: Template: 1HNJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -274664 for 3001 contacts (-91.5/contact) + 2D Compatibility (PS) -32717 + (NN) -7216 + (LL) 520 1D Compatibility (HY) -25200 + (ID) 7500 Total energy: -346777.0 ( -115.55 by residue) QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_1HNJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HNJ-query.scw PDB file : Tito_Scwrl_1HNJ.pdb: