Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLFKSFDASEKTQLICFPFAGGYSASFRPLHAFLQGECEMLAAEPPGHGTNQ-TS-AIEDLEELTDLYKQELNLRPDRPFVLFGHSMGGMITFRLAQKLEREGIFPQAVIISAIQPPHIQ-R-KKVS---HLPD----DQFLDHIIQL----GGMPAE--LVENKEVMSF--FLP--SFRSDYRALEQFEL-YDLAQIQSPVHVFNGLDDKK-CIRDAEGWKKWAKDITFHQFD-GGHMFLLSQTEEVAERIFAILNQHPIIQP 3V48 Chain:A ((8-261)) -----SPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYPA---SVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPRLEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSSELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLL----

General information: TITO was launched using: RESULT: Template: 3V48.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1241 -160188 -129.08 -696.47 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -129.08 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_3V48.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V48-query.scw PDB file : Tito_Scwrl_3V48.pdb: