Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRRKICYCNTALLLMILLAGCTDSKDGEAQQPSNQASAVQTDEKHTEPEESTKIRKDEAEPIT----ESEESATKAANDTSSAEEKSKED--NVLAAYSSEKIEY------------ARVWLQLGPN----------QEIDELNVRHIAAGEPINPNDDTSASYPENVT---QLAGSRLVDGSVTYHGNGDGTIHVY-----NVPLRWDSADDIEKGVMREV-TESIIKNRKTVYVDTGDDEKIKRLIDIMMIH------------------------------------------------------------------- 4RKS Chain:A ((21-337)) --TYRSIGSTAYPTIGVVLLGGIANPVTR---TPLHTSAGIAYSDSCGSIRSETRIYADEATHIYFNGTESTDDNRSVRRVLDRYSSVFEEAFGTKTVSYSSQNFGILSGSSDAGAASIGAAILGLKPDLDPHDVENDLRAVSESAGRSLFGGLTITWSDGFH-AYTEKILDPEAFSGYSIVAFAFDYQRNPSDVIHQNIVRSDLYPARKKHADEHAHMIKEYAKTNDIKGIFDLAQEDTEEYHSILRGVGVNVIRENMQKLISYLKLIRKDYWNAYIVTGGSNVYVAVESENADRLFSIENTFGSKKKMLRIVGGAWHRRPE

General information: TITO was launched using: RESULT: Template: 4RKS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 915 -20372 -22.26 -95.64 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -22.26 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.099

(partial model without unconserved sides chains): PDB file : Tito_4RKS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RKS-query.scw PDB file : Tito_Scwrl_4RKS.pdb: