Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------MIKNGELKPGDKLDSVQALAESFQVSRSAVREALSALKAMGLVEMKQGEGTYLKEFELNQISQPLSAALLMKKEDVKQLLEVRKLLEIGVASLAAEKRTEADLERIQDALKEMGSIEADGELGEKADFAFHLALADASQNELLKHLMNHVSSLLLETMRETRKIWLFSKKTSVQRLYEEHERIYNAVAAGNGAQAEAAMLAHLTNVEDVLSGYFEENVQ 1E2X Chain:A ((10-231)) QSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVTRTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLLSVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPIYGLILNGMKGLYTRIGRHY-----FANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHESGEIWHRMQ-----

General information: TITO was launched using: RESULT: Template: 1E2X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 812 54358 66.94 260.08 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 66.94 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_1E2X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E2X-query.scw PDB file : Tito_Scwrl_1E2X.pdb: