Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSHSVVCIGELLIDFFCTDVD-----VDLMEGRQFLKSAGGAPANVSAAIAKLGGDAAFSGKVGKDPFGYFLKRTLDAVHVDTSMLVMDEKAPTTLAFVSLKQNGERDFVFNRGADALFTLEDIDQ--EKLNEAKILHFGSATALLSDPFCSAYLRLMSIAKDNGQFISFDPNYREDLWRGRVSEFVSVAKKAIAVSDFVKVSDEELEIISGVKD-----HEKGVAILHEIGANIVAVTLGKSGTLLSNGKD--REIIPSIPVTSIDSTGAGDAFVGAALYQLAN--TDQIQSVDADFVKLREIVAFANKVGALVCTKIGAIDALPSLDEIGVSL 2FV7 Chain:A ((24-330)) --VAAVVVVGSCMTDLVSLTSRLPKTGETI-HGHKFFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEIT------PATSLEALTMARRSGVKTLFNPAPAIA----------DLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPNL----------SLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTL

General information: TITO was launched using: RESULT: Template: 2FV7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1832 -193012 -105.36 -663.27 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -105.36 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.467

(partial model without unconserved sides chains): PDB file : Tito_2FV7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FV7-query.scw PDB file : Tito_Scwrl_2FV7.pdb: