Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MKAVILCGGKGTRMSEVTNDIPKPLAMIGGKPILWHIMKIYQYYGVNEFILLLGYKGEKIKEYFLDYEWKHNSLTLDSSTGEVQMLGQPETWKITFLETGVDTLTAGRILQAKDYIGDE-TFLLTYGDG--LANINLFHLISYHQTKGAAATVTGIDKVSQFGTLTVEDGMAKTFSEKTSSD------GIINGGFFVLSPKVFDYLPK------DGNTMFEDEPLKNLAKDGELAVYRHYGFWTAIDTYKNLLEVNKMWNQGQQVWKVW----------------------------------------------------------------------------------- 1FXJ Chain:A ((3-329)) NNAMSVVILAAGKGTRMY---SDLPKVLHTLAGKAMVQHVIDAANELGAAHVHLVYGHGGDLLKQAL-------------------------KDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILMLYGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATDEQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQRLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDTNVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPF

General information: TITO was launched using: RESULT: Template: 1FXJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1186 -162367 -136.90 -718.44 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -136.90 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_1FXJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FXJ-query.scw PDB file : Tito_Scwrl_1FXJ.pdb: