Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIIRIETSRIAVPLTKPFKTALRTVYTAESVIVRITYDSGAVGWGEAPPTLVITGDSMDSIESAIHHVLKPALLGKSLAGYEAILHDIQHLLTGNMSAKAAVEMALYDGWAQMCGLPLYQMLGGYRD-TLETDYTVSVNSPEEMAADAENYLKQGFQTLKIKVGKDDIATDIARIQEIRKRVGSAVKLRLDANQGWRPKEAVTAIRKMEDAGLGIELVEQPVHKDDLAGLKKVTDATDTPIMADESVFTPRQAFEVLQTRSADLINIKLMKAGGISGAEKINAMAEACGVECMVGSMIETKLGITAAAHFAASKRNITRFDFDAPLMLKTDVFNGGITYSG-STISMPGKPGLGIIGAALLKGEKEQ 3Q4D Chain:C ((1-351)) MIITQVELYKSPVKLKEPFKISLGILTHANNVIVRIHTASGHIGYGECSPFMTIHGESMDT-AFIVGQYLAKGLIGTSCLDIVSNSLLMDAIIYGNSCIKSAFNIALYDLAAQHAGLPLYAFLGGKKDKIIQTDYTVSIDEPHKMAADAVQIKKNGFEIIKVKVGGSK-ELDVERIRMIREAAGDSITLRIDANQGWSVETAIETLTLLEP--YNIQHCEEPVSRNLYTALPKIRQACRIPIMADESCCNSFDAERLIQIQACDSFNLKLSKSAGITNALNIIRLAEQAHMPVQVGGFLESRLGFTAAAHVALVSKTICYYDFDTPLMFEADPVRGGIVYQQRGIIEVPETAGLG-------------

General information: TITO was launched using: RESULT: Template: 3Q4D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2147 -197703 -92.08 -566.48 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.81 3D Compatibility (PKB) : -92.08 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_3Q4D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q4D-query.scw PDB file : Tito_Scwrl_3Q4D.pdb: