Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQITDISQLKEAIKQYHSEGKSIGFVPTMGFLHEGHLTLADKA-RQENDAVIMSIFVNPAQFGPNEDFEAYPRDIERDAALAENAGVDILFTPDAHDMYPGEKNVTIHVERRTDVLCGRSREGHFDGVAIVLTKLFNLVKPTRAYFGLKDAQQVAVVDGLISDFFMDIELVPVDTVREEDGLAKSSRNVYLTAEERKEAPKLYRALQTSAELVQAGERDPEAVIKAAKDIIETTSGT-IDYVELYSYPELEPVNEIAGKMILAVAVAFSKARLIDNIIIDIREMERI 1N2I Chain:B ((19-287)) -----DVADVSRALRL---TGRRVMLVPTMGALHEGHLALVRAAKRVPGSVVVVSIFVNPMQFGAGGDLDAYPRTPDDDLAQLRAEGVEIAFTPTTAAMYPDGLRTTVQPGPLAAELEGGPRPTHFAGVLTVVLKLLQIVRPDRVFFGEKDYQQLVLIRQLVADFNLDVAVVGVPTVREADGLAMSSRNRYLDPAQRAAAVALSAALTAAAHAATAGA---QAALDAARAVLDAAPGVAVDYLELRDI-GLGPM-PLNGSGRLLVAARLGTTRLLDNIAIEI------

General information: TITO was launched using: RESULT: Template: 1N2I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1361 -85267 -62.65 -319.35 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -62.65 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_1N2I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N2I-query.scw PDB file : Tito_Scwrl_1N2I.pdb: