Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAVTYQGIKNVVVKDVPDPKIE------KSDDMIIKVTSTAICGSDLHLIHGFIPNMQEDYVIGHEPMGIVEEVGSGVTKLKKGDRVIIPFNIACGECFFCKNQLESQCDQSNDNGEMGAYFGYSGQTGGYPGGQAEYLRVPFANF--THFKIPESCEEPDEK----LSVIADAMTTGFWSVDNAGVKKGDTVIVLGCGPVGLFAQKFCWLKGAKRVIAVDYVNYRLQHAKRTNKVEI-VNFEDHENTGNYLKEITK-GGADVVIDAVGMDGKMSDLEFLASGLKLHGGTMSALVIASQAVRKGGTIQITGVYGGRYN-GFPLGDIMQRNVNIR------------SGQAPVIHYMPYMFELVSTGKIDPGDVVS-HVLPLSEAKHGYDIFDSKMDDCIKVVLKP 1KOL Chain:A ((3-391)) NRGVVYLGSGKVEVQKIDYPKMQDPRGKKIEHGVILKVVSTNICGSDQHMVRGRTT-AQVGLVLGHEITGEVIEKGRDVENLQIGDLVSVPFNVACGRCRSCKEMHTGVCLTVNP-ARAGGAYGYVD-MGDWTGGQAEYVLVP--YADFNLLKLPDR--DKAMEKIRDLTCLSDILPTGYHGAVTAGVGPGSTVYVAGAGPVGLAAAASARLLGAAVVIVGDLNPARLAHAKAQ-GFEIADLSLDTPLHEQIA-ALLGEPEVDCAVDAVGFEA----RGHGHEGAKHE-APATVLNSLMQVTRVAGKIGIPGLYVTEDPGAVDA-AAKIGSLSIRFGLGWAKSHSFHTGQTPVMKYNRALMQAIMWDRINIAEVVGVQVISLDDAPRGYGEFDAGV--PKKFVIDP

General information: TITO was launched using: RESULT: Template: 1KOL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2309 -916 -0.40 -2.54 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -0.40 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_1KOL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KOL-query.scw PDB file : Tito_Scwrl_1KOL.pdb: