Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGNTRKKVSVIGAGFTGATTAFLIAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDAKITGTSNYEDTAGSDIVVITAGIARKPG-----MSRDDLVSTNEKIMRSVTQEIVKYSPDSIIVVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFVAEELNLSVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLETLIPK-----ERIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTEMVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIVGGNGLEQIIELELTDYERAQLNKSVESVKNVMKVLS 3CZM Chain:B ((8-323)) ----RKKIAMIGSGMIGGTMGYLCVLRELADVVLFDV--VTGMPEGKALDDSQATSIADTNVSVTSANQYEKIAGSDVVIITAGLTKVPGKSDKEWSRNDLLPFNAKIIREVAQGVKKYCPLAFVIVVTNPLDCMVKCFHEASGLPKNMVCGMANVLDSARFRRFIADQLEISPRDIQATVIGTHGDHMLPLARYVTVNGFPLREFIKKGKMTEAKLAEIVERTKKAGGEIVRLLGQGSAYYAPALSAITMAQAFLKDEKRVLPCSVYCQGEYGLHDMFIGLPAVIGGGGIEQVIELELTHEEQECFRKSVDDVVELNKSLA

General information: TITO was launched using: RESULT: Template: 3CZM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1797 -60079 -33.43 -196.33 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : -33.43 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_3CZM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CZM-query.scw PDB file : Tito_Scwrl_3CZM.pdb: