Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPDTSMKRVVVKIGSSSLTSLHGEISIRKLEALVDQVVKLKDAGYEVILVSSGAVAAGYRKLGFIQRPEKLPEKQASASIGQGLLMEAYSKLFLAHGYVASQILITRSDFSDEYRYNNVRNTMNVLLERGIIPIINENDTVTVNRLKFGDNDTLAAKVAGLIDADMLVILSDIDGLYDGNPRTNPEAKKIQRVSEITPDIEACAGDTGSIVGTGGMRSKLDAFKIVMASGIKGFLGQADAGDILYHAVHEQAEGTYFEAEGTLPLNQKEQWIAFNSGPEGEMILSDDCSRKITNGQSSLYLDGVQKIKGKFKSGSVVRLMDSKGTEIGLGIVNYSSVQLQEPEKKKELTNRALI--DQEAFVCHVDFSLPVN 4Q1T Chain:A ((5-371)) ---IADSKRLVVKVGSSLVTN---GLDHDAIGRWAAQIAALRNEGKEVVLVSSGAIAEGMQRLGWSRRPREIDELQAAAAVGQMGLAQVYESRFAEHGIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIK--DNDTLGALVANLIEGDALIILTDQQGL------------LVAEASAGAPEL---------------MLTKILAAKRAAHSGANTVIASGRERDVLLRLASGEAIGTQLIART-ARMAARKQWMADHLQVRGHVVIDAGAVDKLTAGGKSLLPIGVVAVQGVFARGEVIACVNDAGREVARGITNYSSAEAKLIQRKPSGEIEAVLGYMLEPELIHRDNLVL--

General information: TITO was launched using: RESULT: Template: 4Q1T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1873 -167962 -89.68 -504.39 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -89.68 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_4Q1T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q1T-query.scw PDB file : Tito_Scwrl_4Q1T.pdb: