Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKDLTGKTAIVTGSSKGIGKAIAERFGKEKMNVVVNYHSDPSGADETLEIIKQN-----GGKAVSVEADVSKEEGIQALLDTALEHFGTLDVMVNNSGFNG-VEA-MPHEMSLEDWQRVIDVNVTGTFLGAKAALNHMMKNNIKGNVLNISSVHQQIPRPVNVQYSTSKGGIKMMTETLALNYADKGIRVNAIAPGTIATE-SNVDTKKEESRQKQLKKIPMKAFGKPEEVAAAAAWLVSEEASYVTGATLFVDGGMTLYPSQLE 2CFC Chain:B ((3-248)) -------RVAIVTGASSGNGLAIATRFLARGDRVAA---LDLSA--ETLEETARTHWHAYADKVLRVRADVADEGDVNAAIAATMEQFGAIDVLVNNAGITGNSEAGVLHTTPVEQFDKVMAVNVRGIFLGCRAVLPHMLLQG-AGVIVNIASVASLVAFPGRSAYTTSKGAVLQLTKSVAVDYAGSGIRCNAVCPGMIETPMTQWRLDQPELRDQVLARIPQKEIGTAAQVADAVMFLAGEDATYVNGAALVMDGAYT-------

General information: TITO was launched using: RESULT: Template: 2CFC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1341 -109872 -81.93 -461.65 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -81.93 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_2CFC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CFC-query.scw PDB file : Tito_Scwrl_2CFC.pdb: