Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVLLGFAAFTLSLSLAACSSNDSEKVSTEKETPQASTDVEKKTEQKESTKEKTADKSKEKDKKELVDVTLDRAVDGDTIKVTYNGNVDTVRYLLIDTPETKKPNSCVQPYGEDASKRNKELV-NSGKLQLEFDKGDRRDKYGRLLAYVYVDGKSVQETLLKEGLARVAYVYEPNTKYIDQFKKDEQEAKSEKLSIWSKNGYVTDRGFNGCVKEKTTAVKKATTSKPAATQPTTPKASSETSTTTEKEASSETTGGTETFKNCTELRKKYPNGVPSSHPAYQSKMDRDHDNYACER 3R3O Chain:A ((13-135)) ----------------------------------------------------------------------TLIKAIDGDTVKLMYKGQPMTFRLLLVDTPEFN------EKYGPEASAFAKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKGNNTHEQLLRKAEAQAKKEKLNIWS--------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3R3O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 601 13712 22.82 112.39 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 22.82 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_3R3O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R3O-query.scw PDB file : Tito_Scwrl_3R3O.pdb: