Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPTEIYVDGASAGNPGPSGIGIFIKHE-GKAE-SFSIPIGVHTNQEAEFLALIEGMKLCATRGYQSVSFRTDSDIVERATELEM-VKNITFQPFVEEIIRLKAAFPLFFIKWIPGKQNQKADLLAKEAIRLNEKN 4E19 Chain:A ((69-198)) -RVHAYFDGASRGNPGPAAVGWVLVSGDGGIVAEGGDTIGRATNNQAEYDALIAALEAAADFGFDDIELRGDSQLVEKQLTGAWDTNDPDLRRKRVRARELLTGFDDWSITHVPRATNERADALANEALDD----

General information: TITO was launched using: RESULT: Template: 4E19.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 666 -10792 -16.20 -84.97 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -16.20 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_4E19.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E19-query.scw PDB file : Tito_Scwrl_4E19.pdb: