Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAVTYQGIKNVVVKDVPDPKIEKSDDMIIKVTSTAICGSDLHLIHGFIPNMQEDYVIGHEPMGIVEEVGSGVTKLKKGDRVIIPFNIACGECFFCKNQLESQCDQSNDNGEMGAYFGYSGQTGGYPGGQAEYLRVPFANF--THFKIPESCEEPDEKLSVIADAMTTGFWSVDNAGVKKGDTVIVLGCGPVGLFAQKFCWLKGAKRVIAVDYVNYRLQHAKRTNKVEI-VNFEDHENTGNYLKEITK-GGADVVIDAVGMDGKMSDLEFLASGLKLHGGTMSALVIASQAVRKGGTIQITGVYGGR-YNGFPL--GDIMQRNVNIRSGQ-APVIHYMPYMFELVSTGKIDPGDVVSHVL-PLSEAKHGYDIFDSKMDDCIKVVLKP 3FPC Chain:A ((1-351)) MKGFAMLSIGKVGWIEKEKP-APGPFDAIVRPLAVAPCTSDIHTVFEGAIGERHNMILGHEAVGEVVEVGSEVKDFKPGDRVVVPAITPDWRTSEVQRGYHQHSGGML-A---GWKFSN-----VKDGVFGEFFHVN--DADMNLAHLPKE--IPLEAAVMIPDMMTTGFHGAELANIKLGDTVCVIGIGPVGLMSVAGANHLGAGRIFAVGSRKHCCDIALEY-GATDIINYKNGDIVEQIL-KATDGKGVDKVVIAGGDV--------------------HTFAQAVKMIKPGSDIGNVNYLGEGDNIDIPRSEWGVGMGHKHIHGGLCPGGRLRMERLIDLVFYKRVDPSKLVTHVFRGFDNIEKAFMLMKDKPKDLIKPVVIL

General information: TITO was launched using: RESULT: Template: 3FPC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2132 -89942 -42.19 -262.99 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -42.19 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_3FPC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FPC-query.scw PDB file : Tito_Scwrl_3FPC.pdb: