Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILVLAVHPHMETSVVNKAWAEELSKHDNITVRDLYKEYPDEAIDVAKEQQLCEEYDRIVFQFPLYWYSSPPLLKKWQDLVLTYGWAFGSEG----NALHGKELMLAVSTGSEAEKYQAGGANHYSISELLKPFQ-ATSNLIGMKYLPPYVFYGVNYAAAEDISHSAKRLAEYIQQPFV 4U7F Chain:A ((81-199)) -------------------------------------------ASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTGVNGDS-RYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEE-----------------

General information: TITO was launched using: RESULT: Template: 4U7F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 450 -23945 -53.21 -210.04 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -53.21 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_4U7F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U7F-query.scw PDB file : Tito_Scwrl_4U7F.pdb: