Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYIMKQSGWLELICGSMFSGKSEELIRRVKRATYAKQEVRVFKPVIDNRYSEAAVVSHNGTSMTSYAISSAADIWD-----HISESTDVVAVDEVQFFDQEIVEVLSSLADKGYRVIAAGLDMDFRGEPFGVVPNIMAIAESVTKLQAVCSVCGSPASRTQRLIDGKPASYDDPVILVGAAESYEARCRHHHEVPGKSKK 2B8T Chain:A ((11-200)) ------IGWIEFITGPMFAGKTAELIRRLHRLEYADVKYLVFKPKIDTRSI-RNIQSRTGTSLPSVEVESAPEILNYIMSNSFNDETKVIGIDEVQFFDDRICEVANILAENGFVVIISGLDKNFKGEPFGPIAKLFTYADKITKLTAICNECGAEATHSLRKIDGKHADYNDDIVKIGCQEFYSAVCRHHHKVPNR---

General information: TITO was launched using: RESULT: Template: 2B8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 882 -21090 -23.91 -114.00 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -23.91 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_2B8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B8T-query.scw PDB file : Tito_Scwrl_2B8T.pdb: