Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------MEERYSRQIRFKQIGEEGQKRLADSHVLIVGAGALGTAGAEGLSRAGVGTITIIDRDYVEWSNLQRQQLYTESDAKLRMPKAMAAKEHLSAINSEIHIEAYVTEGTAETLEPLIEKADVVIDATDNFETRMLINDLAQ------------KTKTPWVYGACVSSQGMFMTVIPGETPCLSCLFEQIPVGG--ATCDTAGIIPPAVHIVSAYQQ-------------------AEALKLLTGQKEAIQRGFVTFDVWNNSHMKINVNHV--RREECPSCGANAVYPYLQDWNT------PKAAVLCGRDTVQVRSESLKRIPK-------------------QELIKRLKTIGK-------VEANAFLLHIFYEDFRIVIFNDGRALVHGTNDVKEANSVLARVIGL--- 1R4M Chain:B ((1-430)) DWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKD--IGRPKAEVAAEFLNDRVPNCNVVPH-FNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMATIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGV-TYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATNRTLYLQSVTSIEERTRKELGLVDGQEAVADVTTPQTVLFKLHFT

General information: TITO was launched using: RESULT: Template: 1R4M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1555 -174197 -112.02 -519.99 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -112.02 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.384

(partial model without unconserved sides chains): PDB file : Tito_1R4M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1R4M-query.scw PDB file : Tito_Scwrl_1R4M.pdb: