Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVLLGFAAFTLSLSLAACSSNDSEKVSTEKETPQASTDVEKKTEQKESTKEKTADKSKEKDKKELVDVTLDRAVDGDTIKVTYNGNVDTVRYLLIDTPETKKPNSCVQPYGEDASKRNKELV-NSGKLQLEFDKGDRRDKYGRLLAYVYVDGKSVQETLLKEGLARVAYVYEPNTKYIDQFKKDEQEAKSEKLSIWSKNGYVTDRGFNGCVKEKTTAVKKATTSKPAATQPTTPKASSETSTTTEKEASSETTGGTETFKNCTELRKKYPNGVPSSHPAYQSKMDRDHDNYACER 3MXP Chain:A ((13-135)) ----------------------------------------------------------------------TLIKAIDGDTVKLMYKGQPMTFRLLLVDTPEFN------EKYGPEASAFAKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKGNNTHEQLLRKAEAQAKKEKLNIWS--------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3MXP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 604 9872 16.34 80.92 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 16.34 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_3MXP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MXP-query.scw PDB file : Tito_Scwrl_3MXP.pdb: