Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQFDKQYNSIIKDIINNGISDEEFDVRTKWDSDGTPAHTLSVMSKQMRFD-NSEVPILTTKKVAWKTAIKELLWIWQLKSNDVNDLNKMGVHIWDQ------------WKQEDGTIGHAYGFQ---LGKKNRSLN----GEKVDQVDYLLHQLKNNPSSRRHITMLWNPDDLDAMSLTPCVYETQWYVKHGKLHLEVRARSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEAPELWINPEVKDFYNFTIDDFKLINYKHGDKLLFEVAV 2TSR Chain:B ((26-301)) ------QYLRQVEHIMRCGFKKE----------DRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIK-GSTNAKELSSKGVRIWDANGSRDFLDSLGFSARQEGDLGPVYGFQWRHFGADYKDMDSDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVETIDDFKVEDFQIEGYNPHPTI------

General information: TITO was launched using: RESULT: Template: 2TSR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1225 12535 10.23 48.96 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : 10.23 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.624

(partial model without unconserved sides chains): PDB file : Tito_2TSR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2TSR-query.scw PDB file : Tito_Scwrl_2TSR.pdb: