Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLDAVTFGESMAMFYANEYGGLHEVSTFSKGLAGAESNVACGLARLGF--RMGWMSKVGNDQLGTFILQELKKEGVDVSRVIRSQDENPTGLLLKSKVKEGDPQVTYYRKNSA-ASTLTTA--EYPRDYFQCAGHLHVTGIPPAL-SAEMKDFTYHVMNDMRNAGKTISFDPNVRPSLWPDQATMVHTINDLAGLADWFFPGIAEGELLTGEKTPEGIADYYLKKGASFVAIKLGKEGAYFKTGTSEGFLEGCRVDRVVDTVGAGDGFAVGVISGILDGLSYKDAVQRGNAIGALQVQAPGDMDGLPTREKLASFLSAQRTVHQKKGDY 4E69 Chain:A ((22-319)) SMMHILSIGECMAELAPAD-----LPGTYRLGFAGDTFNTAWYLARLRPESRISYFSAIGDDALSQQMRAAMSAAGIDG-GGLRVIPGRTVGLYLITLE-QGERSFAYWR-GQSAARELAGDADALA-AAMARADVVYFSGITLAILDQCGRATLLRALAQARATGRTIAFDPNLRPRLWAGTGEMTETIMQGAAVSDIALPSFEDEAAWFGDAGPDATADRYARAGVRSVVVKNGPHAVHFLQDGRRGRVPVPPVAQVVDTTAAGDSFNAGLLDSVLAGQPLETAIAAAAALAGQVVQGKGALVEV-----------------------

General information: TITO was launched using: RESULT: Template: 4E69.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1692 -11232 -6.64 -38.47 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -6.64 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_4E69.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E69-query.scw PDB file : Tito_Scwrl_4E69.pdb: