Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVITDITELIGNTPLLRLKNFDVPEGVAVYAKLEMMNPGGSIKDRLGDMLIRDALDSGKVKPGGVIIEATAGNTGIGLALSARKYGLKAIFCVPEHFSREKQQIMQALGASIIHTPRQDGMQGAIQKAIQLETEIENSYCVL-QFKNRVNPSTYYKTLGPEMWEALDGNIHTFVAGAGSGGTFAGTASFLK--EKNPAVKTVIVEP-----VGSILNGGE--PHAHKTEGIGMEFIPDYMDKSHFDEIYTVTDENAFRLVKEAAEKEGLLIGSSSGAALYAALE-EAKKASAGTNIVTVFPDSSDRYISKQIYEGGI 1FCJ Chain:D ((11-302)) ----------IGHTPLVRLNRIG---NGRILAKVESRNPSFSVKCRIGANMIWDAEKRGVLKPGVELVEPTNGNTGIALAYVAAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGMKGAIQKAEEIVASDPQKYLLLQQFSNPANPEIHEKTTGPEIWEDTDGQVDVFISGVGTGGTLTGVTRYIKGTKGKTDLITVAVEPTDSPVIAQALAGEEIKPGPHKIQGIGAGFIPGNLDLKLIDKVVGITNEEAISTARRLMEEEGILAGISSGAAVAAALKLQEDESFTNKNIVVILPSSG-------------

General information: TITO was launched using: RESULT: Template: 1FCJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1599 -6593 -4.12 -23.46 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -4.12 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_1FCJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FCJ-query.scw PDB file : Tito_Scwrl_1FCJ.pdb: