Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIVKATDQSFSAET--SEGVVLADFWAPWCGPCKMIAPVLEELDQEMGDKLKIVKIDVDENQETAGKYGVMSIPTLLVLKDGEVVETSVGFKPKEALQELVNKHL 2H70 Chain:B ((4-107)) --IIHLTEDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANL

General information: TITO was launched using: RESULT: Template: 2H70.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 436 14117 32.38 138.40 target 2D structure prediction score : 0.86 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : 32.38 2D Compatibility (Sec. Struct. Predict.) : 0.86 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.665

(partial model without unconserved sides chains): PDB file : Tito_2H70.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H70-query.scw PDB file : Tito_Scwrl_2H70.pdb: