Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLISIDSRKHLFYEEYGQG-IPIIFIHPPGMGRKVFYY-QRLLSKHFRVIFPDLSGHGDSDHIDQPASISYYANE-IAQFMDALHIDKAVLFGYSAGGLIAQHIGFTRPDKVSHLILSGAYPAVHN------VIGQKLHKLGMYLLEKNPGLLMKILA----GSHTKDRQLRSILTDHMKKA-DQAHWHQYYLDSLGYNCIEQLPRLEM-PMLFMYGGLRDWTFTNAGY-YRRSCRHAEFFRLEYQGHQLPTKQWKTCNELVTGFVLTHHS 1M33 Chain:A ((3-258)) ----------NIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHLVDLPGFGRSRGFGALS-----LADMAEAVLQQA-PDKAIWLGWSLGGLVASQIALTHPERVRALVTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDQQRTVERFLALQTMGTETARQDARALKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPMLDKLWPHSESYIFAKAAHAPFISHPAEFCHLLVALKQRVGS

General information: TITO was launched using: RESULT: Template: 1M33.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1382 -33860 -24.50 -141.08 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -24.50 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.362

(partial model without unconserved sides chains): PDB file : Tito_1M33.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M33-query.scw PDB file : Tito_Scwrl_1M33.pdb: