Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVDYEREEEHTTPEQPKRSKKGYFLSSLIGVIVGAVLMAFIMPYLSNEGLDTGALDQQQNNNGRESIRTVNVSVNNAVTKIVSNMSPAVVGVVNIQKSD-IWGE---SGEAGSGSGVIYKKNDHSAYVVTNHHVIEGASQIEISLKDGSRVSADLVGSDQLMDLAVLRVKSDK--IKAVADFGNSDKVKSGEPVIAIGNPLGLEFAGSVTQGVISGTERAIPVDSNGDGQPDWNAEVLQTDAAINPGNSGGALLNMDGKVIGINSMKIAESA-VEGIGLSIPSKLVIPVIEDLERYGKVKRPFLGIEMKSLSDIASYHWDETLKLPKNVTNGAVVMGVDAFSPAGKAGLKE-----------LDVITEFDGYKVNDIVDLRKRLYQKKVGDRVKVKFYRGGKEKSVDIKLSSADQLGS 3QO6 Chain:A ((14-334)) ------------------------------------------------------------------------TDELATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDK---QGHIVTNYHVIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKNKL-RPIPVGVSADLLVGQKVFAIGNPFGL--DHTLTTGVISGLRREISSAAT----GRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYSPSGASSGVGFSIPVDTVGGIVDQLVRFGKVTRPILGIKFAPDQSVE------QL-----GVSGVLVLDAPPSGPAGKAGLQSTKRDGYGRLVLGDIITSVNGTKVSNGSDLYRILDQCKVGDEVTVEVLRGDHKEKISVTLEPKP----

General information: TITO was launched using: RESULT: Template: 3QO6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1650 42997 26.06 141.90 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 26.06 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_3QO6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QO6-query.scw PDB file : Tito_Scwrl_3QO6.pdb: