Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNDKTAIVTGASRGIGRSIALDLAKSGANVVVNYSGNEAKANEVVDEIKSMGRKAIAVKADVSNPEDVQNMIKETLSVFSTIDILVNNAGITRDNLIMRMKEDEWDDVININLKGVFNCTKAVTRQMMKQRSGRIINVSSIVGVSGNPGQANYVAAKAGVIGLTKSSAKELASRNITVNAIAPGFISTDMTDKLAKDVQDEMLKQIPLARFGEPSDVSSVVTFLASEGARYMTGQTLHIDGGMVM 4BO3 Chain:C ((25-268)) -LQGKVALVTGASRGIGQAIALELGRLGA-VVIGTATSASGAEKIAETLKANGVEGAGLVLDVSSDESVAATLEHIQQHLGQPLIVVNNAGITRD--------DEWFDVVNTNLNSLYRLSKAVLRGMTKARWGRIINIGSVVG---NAGQTNYAAAKAGLEGFTRALAREVGSRAITVNAVAPGFIDTDMTRELPEAQREALLGQIPLGRLGQAEEIAKVVGFLASDGAAYVTGATVPVNGGMYM

General information: TITO was launched using: RESULT: Template: 4BO3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1210 -149193 -123.30 -640.31 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.85 3D Compatibility (PKB) : -123.30 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_4BO3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BO3-query.scw PDB file : Tito_Scwrl_4BO3.pdb: