Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQMNRYDQAATLRAKMEKRERVLPMVYSQKAKTLAVISGKGGVGKSNITLNMALAL-QDKGKKVLLIDLDIGMGNID-ILIGNSSSATIIDVLTDRK----PLLQS-LSVGPKGLRYISGGTGLDVMFQLDQRKWTFFANELSHALSQFDYVLFDMGAGLSKDQLPFILSAEDILIITTPEPTAIMDAYSAVKHLVLTENKLS-MKVAVNRCRDQKEGLDAFARLSRTIHMFLDVQVQFAGSVSDDVIV-SKAVVEQVPFFIKSPQAKASRSVRILADALFEREETRHKEDKQTFIEKLSSFLMRRA 3EA0 Chain:A ((3-242)) -----------------------------AKRVFGFVSAKGGDGGSCIAANFAFALSQEPDIHVLAVDISLPFGDLDMYLSGNTHSQDLADISNASDRLDKSLLDTMVQHISPSLDLIPSPATFEKIVNIEPERVSDLIHIAASF---YDYIIVDFGASIDHVGVWVLEHLDELCIVTTPSLQSLRRAGQLLKLCKEFEKPISRIEIILNR--ADTNSRITSDEIEKVIG------RPISKRIPQDEDAMQESLLSGQSVLKVAPKSQLSKTIVDWALHL---------------------------

General information: TITO was launched using: RESULT: Template: 3EA0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1254 -161773 -129.01 -700.31 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -129.01 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_3EA0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EA0-query.scw PDB file : Tito_Scwrl_3EA0.pdb: