Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGNTRKKVSVIGAGFTGATTAFLIAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDAKITGTSNYEDTAGSDIVVITAGIARKPG-----MSRDDLVSTNEKIMRSVTQEIVKYSPDSIIVVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFVAEELNLSVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLETLIPK-----ERIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTEMVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIVGGNGLEQIIELELTDYERAQLNKSVESVKNVMKVLS 1SOW Chain:A ((8-323)) ----RKKIAMIGSGMIGGTMGYLCVLRELADVVLFDV--VTGMPEGKALDDSQATSIADTNVSVTSANQYEKIAGSDVVIITAGLTKVPGKSDKEWSRNDLLPFNAKIIREVAQGVKKYCPLAFVIVVTNPLDCMVKCFHEASGLPKNMVCGMANVLDSARFRRFIADQLEISPRDIQATVIGTHGDHMLPLARYVTVNGFPLREFIKKGKMTEAKLAEIVERTKKAGGEIVRLLGQGSAYYAPALSAITMAQAFLKDEKRVLPCSVYCQGEYGLHDMFIGLPAVIGGGGIEQVIELELTHEEQECFRKSVDDVVELNKSLA

General information: TITO was launched using: RESULT: Template: 1SOW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1797 -60672 -33.76 -198.27 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -33.76 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.562

(partial model without unconserved sides chains): PDB file : Tito_1SOW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SOW-query.scw PDB file : Tito_Scwrl_1SOW.pdb: