Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MKKVRKSFVLLLTGLLAVLILSACGQKTQQDIVAGLDEKAKEYTSYKAKAKMTIETGSEPQVYNVEIWHKKPSLYRVYLENPKKDQNQVILRNENGVFVLTPSLNKSFRFQSDWPNNSSQVYLFESLVKDVQNDSDAVFTAKEKKYVFETKTNYQHNKMLPTQEITFNKKDMSPSSVKVMDTDRKVMVKVEFSSFEFNKQFDKESFDEKKNMTLSQMDVATSAKPSDTFAVKTPLELPLGVKLLEEKDISTEDGKRIIMTYGGEKSFTLIQEKAQIAKASSSVTLNGEPVNLGYTIGALSDASLSWTYDGVDYLLSSKDLSKEEMVTVAKSMQGQSSK 4P0P Chain:B ((62-389)) RMKAQRPE--ECLKHIIVVLDPVLLQMEGGGQLLGALQTM---------ECRCVIE---AQAVPCSVTWRRRA--------GPSEDRED--WVEEPTVLVLLR----AEAFVSMIDNGKTLQGFVTDITAKTAGKALSLVIVDQEK---------CFVSRVDAEEALVDLQLHTEAQAQIVQSWKEL---ADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAY---------PSPQLLVQAYQQCFSDKERQNL------LADIQVRRTSRRIGPELSRRIYLQMT------TLQPHLSL

General information: TITO was launched using: RESULT: Template: 4P0P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1054 -83131 -78.87 -300.11 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -78.87 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.249

(partial model without unconserved sides chains): PDB file : Tito_4P0P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P0P-query.scw PDB file : Tito_Scwrl_4P0P.pdb: