Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKSKVAKELKRQQLVEQYAGIRRELKEKGDYEALSKLPRDSAPGRLHNRCMVTGRPRAYMRKFKMSRIAFRELAHKGQIPGVKKASW 2UUA Chain:N ((19-61)) ----------------------------------------------RAYTRCVRCGRARSVYRFFGLCRICLRELAHKGQLPGVRKASW

General information: TITO was launched using: RESULT: Template: 2UUA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 113 -29139 -257.86 -677.64 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain N : 0.75 3D Compatibility (PKB) : -257.86 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.657

(partial model without unconserved sides chains): PDB file : Tito_2UUA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2UUA-query.scw PDB file : Tito_Scwrl_2UUA.pdb: