Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANQKKKTLPPQHQNQQPGFEYLMDPRPVFDKPKKAKKLEGKTAIITGGDSGIGRAVSVLFAKEGANVVIVYLNEHQDAEETKQYVEKEGVKCLLIAGDVGDEAFCNDVVGQASQVFPSIDILVNNAAEQHVQPSIEKITSHQLIRTFQTNIFSMFYLTKAVLPHL--KKGSSIINTASITAYKGNKTLIDYSATKGAIVTFTRSLSQSLVQQGIRVNAVAPGPIWTPLIPASFAAKDVEVFGSDVPMERPGQPVEVAPSYLYLASDDSTYVTGQTIHVNGGTIVNG 4WJZ Chain:B ((10-247)) --------------------------------------LEGKVALVTGASRGIGKAIAELLAERGAKVIGTATSES-GAQAISDYLGDNGKGMAL---NVTNPESIEAVLKAITDEFGGVDILVNNA----------------WSDIMETNLTSIFRLSKAVLRGMMKKRQGRIINVASVVG-------ANYAAAKAGVIGFTKSMAREVASRGVTVNTVAPGFIETDMT-----EQRTATL-AQVPAGRLGDPREIASAVAFLASPEAAYITGETLHVNGG-----

General information: TITO was launched using: RESULT: Template: 4WJZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1120 -140231 -125.21 -670.96 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -125.21 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_4WJZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WJZ-query.scw PDB file : Tito_Scwrl_4WJZ.pdb: