Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQFDKQYNSIIKDIINNGISDEEFDVRTKWDSDGTPAHTLSVISKQMRFD-NSEVPILTTKKVAWKTAIKELLWIWQLKSNDVNDLNMMGVHIWDQ------------WKQEDGTIGHAYGFQ---LGKKNRSL----NGEKVDQVDYLLHQLKNNPSSRRHITMLWNPDELDAMALTPCVYETQWYVKHGKLHLEVRARSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEAPELWINPEVKDFYDFTIDDFKLINYKHGDKLLFEVAV 2TSR Chain:A ((26-301)) ------QYLRQVEHIMRCGFKKE----------DRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIK-GSTNAKELSSKGVRIWDANGSRDFLDSLGFSARQEGDLGPVYGFQWRHFGADYKDMDSDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVETIDDFKVEDFQIEGYNPHPTI------

General information: TITO was launched using: RESULT: Template: 2TSR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1204 10803 8.97 42.20 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 8.97 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_2TSR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2TSR-query.scw PDB file : Tito_Scwrl_2TSR.pdb: