Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDVIKISPRGYCYGVVDAMVIAKNASLDKSLPRPIYILGMIVHNKHVTDAFEEEGIFTLDGSNRLDILKQVDKGTVIFTAHGVSPEVRRIAEEKGLVAIDATCPDVTKTHNLILEMKEKGYHVIYIGKKGHPEPEGAVG--VAPE-IVHLVETEEDVKNLDIQS-EKLIVTNQTTMSQWDVHDIMELVKEKYPHV--EYHQEICLATQVRQEAVSEQAKKADLTIVVGDPKSNNSNRLAQVSEEIAGTKAYRIGDLSELKLEWLKGVNTVAVTAGASTPTPITKEVIRFLEQFDHEDPSTWTTEHNIPLKKILPKVKAKN 3T0G Chain:A ((13-297)) MQILLANPRGFCAGVDRAISIVENAL--AIYGAPIYVRHEVVHNRYVVDSLRERGAIFIE-----QISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKVHMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEKLSFMTQCTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVLVVGSKNSSNSNRLAELAQRM-GKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQNVVARLQQL---------------------------

General information: TITO was launched using: RESULT: Template: 3T0G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1577 -25038 -15.88 -89.74 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -15.88 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_3T0G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T0G-query.scw PDB file : Tito_Scwrl_3T0G.pdb: