Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MDSKDHRNELNRFFKSFVHAGRGIWETARTERNFQFHAAAACAVLICGFLVELSIIEWMIIFLLIGGMFSLELLNTAIEHTVDLITDKHHPLAKAAKDAAAGAVCVFAVISCIIGLLIFLPKL 2P61 Chain:A ((34-153)) EFFDILEDVKEDHFEKLLEEAVEEVIDSGNELVRSPTPSNLKRYKNAIKEFLKLIEKKIY-----------------KLNSGRARLHLVVEEVNEKLMDLTEKIMKNEWQTINLAARIEEINGLILNLYRE

General information: TITO was launched using: RESULT: Template: 2P61.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 231 -16473 -71.31 -155.41 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -71.31 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_2P61.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P61-query.scw PDB file : Tito_Scwrl_2P61.pdb: