Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVNPITHYIGSFIDEFALSGITDAVVCPGSRSTPLAVLCAAHPDISVHVQIDERSAGFFALGLAKAKQRPVLLICTSGTAAANFYPAVVEAHYSRVPIIVLTADRPHELREVGA-PQAINQHFLFGNFVKFFTDSALPEESPQMLRYIRTLASRAAGEAQKR-PMGPVHVNVPLREPLMPDLSDEPFGRMRTGRHVSVKTGTQSVDRESLSDVAEMLAEAEKGMIVCGELHS-DADKENIIALSKALQYPILAD-PLSNLRNGVHDKSTVIDAYDSFLKDDELKRKLRPDVVIRFGPMPVSKPVFLWLKDD-PTIQQIVIDEDGGWRDPTQASAHMIHCNASVFAEEIMAG-LTAATRSSEWLEKWQFVNGRFREHLQTISSEDVSFEGNLYRILQHLVPENSSLFVGNSMPI-RDVDTFFEKQDRPFRIYSNRGANGIDGVVSSAMGVCEGT----K-APVTLVIGDLSFYHDLNGLLAAKKLGIPLTVILVNNDGGGIFSFLPQASEKTHFEDLFG-TPTGLDFKHAAALYGGTYSCPASWDEFKTAYAPQADKPGLHLIEIKTDRQSRVQLHRDMLNEAVREVKKQWEL 4Q9D Chain:A ((4-532)) -----QKTVHDVTYDLLRKLGLTTVFGNPGSTEESFLRDFP--EDFTYVLSLQEASALAMADGFAQATGKPALVNLHTAAGTGNAMGSLVAAYRANTPLIITAGQQTREMSVVDPYLNNPDATTMPKPWVKWSYEPARAE-------DVPAAFMQAYAVA-MQPPMGPVFLSIPLDDWDKPALGPAA----V-RS---VSTR-VAPDAERLAQFAERINAAKHPMLVLGPEVDRAGAWDAGIEFAEKLGAPVHASALPDRMSFPED-HPLYAGPLPMT-IAGVEQAVSAYDLVVVVGAEVFRYYPYVPGEYLPEGTDLLQITADPHRSA-VAPVGDSLVGDVGIALSRLTELIDTPD-DRVPPKPLVRQRH-------SDIPSTAPMTSNAVYEVLSNVKPDDAAVVMESTSTMLDLFTWL--PTTHPASFF-ATGSGGIGWGVPAAVGIALGDRARGVDRTVVATIGDGSFQYSIQAIWTAAQHKLPIVFVVLRNGEY---------------PNVPGLQLPGLDISSIAAGFGCRTATVESTDMLEAELKTALQADGPTVLVVPTLPQ-----------------------

General information: TITO was launched using: RESULT: Template: 4Q9D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3087 -65908 -21.35 -130.77 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -21.35 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.400

(partial model without unconserved sides chains): PDB file : Tito_4Q9D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q9D-query.scw PDB file : Tito_Scwrl_4Q9D.pdb: