Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIKEAGPFPVKKEQFRMTVNGQ-AWEVAAVPTTHLSDLLRKEFQLTGTKVSCGIGRCGACSILID------GKL----ANACMTMAYQADGHSITTIEGL--QKEELDMCQTAFLEEGGFQCGYCTPGMIIALKALFRETPQPSDKDIEEGLAGNLCRCTGYGGIMRSACRIRRELNGGRRESGF 1FIQ Chain:A ((10-158)) -------------------VNGKKVVEKNADPETTLLAYLRRKLGLRGTKLGCGEGGCGACTVMLSKYDRLQDKIIHFSANACLAPICTLHHVAVTTVEGIGSTKTRLHPVQERIAKSHGSQCGFCTPGIVMSMYTLLRNQPEPTVEEIEDAFQGNLCRCTGYRPILQ------------------

General information: TITO was launched using: RESULT: Template: 1FIQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 575 -2423 -4.21 -17.82 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -4.21 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_1FIQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FIQ-query.scw PDB file : Tito_Scwrl_1FIQ.pdb: