Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSERTFNWKNKDIRAQVDVVDSKLLPTLLLRNALVLNPYVKQWLKKNIWIYQDRIVYVGHELPNR--AEEIHTIDCEGKYIVPGYIEPHAHPF-QIYNP------------------QTLAEYVSQYGTTTFVNDNLFLLLQSGKKKALTILNELKKQPVQYFWWSRYDLQTE------------------------VLN---ED-HVLPFDVRKQWIEH----P-DVIQG---GEMT---GWPR-LVDGDDLMLHCMQATKKQRKRIEGHFPGASDKTLTKMKLFGAD-CDHEAMTGDEVMRRLE-LGYYVSLRNSSI--R--------------------PDVRKILQELHEKGFRYYDHFFYTTDGATPNFYKGGMTNELIRIAL-EEGVPAIDAYNMASFNIAKYYQMD-DYLGVVGPGRLASLNILE-DPLN--------PNPVTVLSKGTILRENGCDLKAFTKTDWHKGGLVPLELSYDMTMDDLQFSMPMGVKMRNAVIMEPYMIEIDNSMEQLSFDHDESYLTMLDRHGKWRVNTMIKGFASSVQGFVSSFTTTGDIVAIGKNKADMLLAFARMKEIGGGIVLAENGNILHEIPLALCGCASSEAYEDVLEKEQKLRDLLTERGYEFCDPIYTLLFLQSTHLPYIRITPRGIFDVMKKTVLFPSIMR 4C65 Chain:A ((45-470)) ----------------------DEAKVTIIYAGLLIPGDGEPLRNAALVISDKIIAFVGSEADIPKKYLRSTQSTHRVPVLMPGLWDCHMHFGGDDDYYNDYTSGLATHPASSGARLARGCWEALQNGYTSYRDLAG-Y----G-CEVAKAINDGTIVGPNVYSSGAALSQTAGHGDIFALPAGEVLGSYGVMNPRPGYWGAGPLCIADGVEEVRRAVRLQIRRGAKVIKVMASGGVMSRDDNPNFAQFSPEELKVIVEEAARQNRIVSAHVHGKA--GIMAAIKAGCKSLEHVSYADEEVWELMKEKGILYVATRSVIEIFLASNGEGLVKESWAKLQALADSHLKAYQGAIKAG-V---TIALGTDTAPGG-----PTALELQFAVERGGMTPLEAIKAATANAPLSVGPQAPLTGQLREGYEADVIALEENPLEDIKVFQEPKAVTHVWKGGKLFKGPGIGP-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4C65.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2041 -157266 -77.05 -476.56 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -77.05 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.366

(partial model without unconserved sides chains): PDB file : Tito_4C65.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C65-query.scw PDB file : Tito_Scwrl_4C65.pdb: