Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKTLALAATAAVLMLSACSSGFGGEKEEEITQKTAKSSEKAIVPKYNISDSYYKMVLPFKAGKARGLTTEQL-NTRLDIDEFETGLMRLAQDSFSTDDYLFQEGQYLDEDTVLSWLARKKTGSDLKK-AEKEDKNF---KNEGLNPALPSSGSTEEKNESSPIYLASMLEHDYLVRKDKN-SIQLGGVMIGLAL-NSVYYYREKTGDPQKEVEIKDSTLRQQGEKIAQEVINRLRKKDNLKNVPITVALYKQASKTSIVPGNFIAKTEVKAGSTDISNWDDINEKYVFYPADTTTAEKYPDDTEVFKRFKNSIEEYFPNYTGVVGTALYENDEMKKMKIDIPMQFYGKSEVVAFTQFLTGEVMDYYSKSSVDVEVNITSSDGQEAVIIRNAGDKEPTVHIYD- 2QX2 Chain:A ((13-344)) --------------------------------------------------------------------LLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQDGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETNPEKIAEKSPAYLSNILEQDFYGKNIKGMTIGLA--------MNSVYYYKKEKDGPTFSKKLDDSEVKKQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKL-EWHNINEKSALLPS--------STAADYDENLNNNFKQFNDNLQQAVGKVKFVDKKPQRLVVDLPIDYYGQAETIGITQYVTEQANKYFDKI-DNYEIRIKDGNQPRALISKTKDDKEPQVHIYSN

General information: TITO was launched using: RESULT: Template: 2QX2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1477 -40215 -27.23 -129.73 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -27.23 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_2QX2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QX2-query.scw PDB file : Tito_Scwrl_2QX2.pdb: