Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAHRILIVDDAAFMRMMIKDILVKNGFEVVAEAENGAQAVEKYKEHSPDLVTMDITMPEMDGITALKEIKQIDAQAR--IIMCSAMGQQSMVIDAIQAGAKDFIVKPFQADRVLEAINKTLN 3RVS Chain:B ((10-125)) ---KFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWDMPNMDGLELLKTIRADGAMSALPVLMVTARAKKENIIAAAQAGASGYVVKPFTAATLEEKLNK---

General information: TITO was launched using: RESULT: Template: 3RVS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 535 -69557 -130.01 -610.15 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -130.01 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_3RVS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RVS-query.scw PDB file : Tito_Scwrl_3RVS.pdb: