Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGGMALTGNKAVVYKGKGTVAVEDIGYPELILRDGPGVPKANVNRKCEHGVILKVITTNICGSDQHMVRGR-TTAPEGLVLGHEITGEVIETGRDVEFIKKGDIVSVPFNIACGRCVMCKTQKTHVCLNVNPDRPGS---------AYGYVDMGGWVGGQ--SEYVMVPYADFQLLVFPDKEQALEKILDLTMLSDIFPTGFHGAY-TAGVQTGSTVYIAGAGPVGLAAAHSAQLLGASTVIVGDLNEDRLAQARSFGC-ETVNVQKHDRLGEQIEQILGEPTVDAAVDCVGFEASGHGNQGEAPAAVLNSIMDVTQVGGSLGIPGLYVTEDPGAKDADAKTGSLKIRFGLGWAKAHTFVTGQTPAMTYNRNLMKAILSGRAQIAKAVNATVISLDDAPKGYSDFDKGAAKKFVIDPHGTLK 1TEH Chain:A ((1-373)) -ANEVIKCKAAVAWEAGKPLSIEEIEVAP---------PKA-------HEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADIS---VAKIDPLAPLDKVCLLGC---GISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIG------------NVKVMRAALEACHKGW-----GVSVVVGVAASGEEIATRPFQLVTGRTWKGTAF---GGWKSVESVPKLVSEYMSKKIKVDEFVTHNLS-FDEINKAFELMHSGKSIRTVVKI-----

General information: TITO was launched using: RESULT: Template: 1TEH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2237 -84348 -37.71 -234.95 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -37.71 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.374

(partial model without unconserved sides chains): PDB file : Tito_1TEH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TEH-query.scw PDB file : Tito_Scwrl_1TEH.pdb: