Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLISWNVNGLRAVMRKMDFLSYLKEEDADIICLQETKIQDGQVDL---QPEDYHVYWNYAVKKGYSGTAVFSKQEPLQVIYGIGVEEHDQEGRVITLEFENVFVMTVYTPNSRRGLERIDYRMQWEEALLSYILEL-DQKKPVILCGDLNVAHQEIDLKNPKANRNNAGFSDQEREAFTRFLEA-GFVDSFRHVYPDLEGAYSWWSYRAGARDRNIGWRIDYFVVSESLKEQIEDASISADVMGSDHCPVELIINI 4B5F Chain:A ((2-250)) LKIISANVNGIRSAYKK-GFYEYIAASGADIVCVQELKAQEADLSADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGRLSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLKNWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPG-YTWWSNRGQAYAKDVGWRIDYQMVTPELAAKAVSAHVYKDEKFSDHAPL------

General information: TITO was launched using: RESULT: Template: 4B5F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1305 5863 4.49 24.03 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : 4.49 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.578

(partial model without unconserved sides chains): PDB file : Tito_4B5F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4B5F-query.scw PDB file : Tito_Scwrl_4B5F.pdb: