Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPINSFENYPMSWKPSIDKAEKPIYKALAGQLEQDILNGVLLPGTKLPPQRELADYLDLNVSTISKAFKVCELKGLLSATVGSGTFVSYDALSNAYLLEDTKPTHLIEMGAILPDHASYE---PLLYQLKNMVHEEDYEKWFSYGRAGESLWQKDAAVKLIQR-GGFETSVDHILFANGGQNAIAATLASLCKPGDRIGVDHHTYPGLKTVASLLSVQIVPIKSEND---EMSPESLEYACKNENIKGLYLIPDYHNPTTSFMSVENRRMVADIVKKYNLFVIEDASYHLLNKNP--LPALASFA-PQQVIHIASLSKSLA-PGLRLAYVAVPRQ------YKEPISKALYNLNITVSPLLAELTARTIVSN-QFEVLIESHREQTIRRNQLVNRYVAGY---TCLGVETGIFRWLLLPGKM-----SGAEFEELAARLGVQVYAAERFVVGNSCPERAVRVSVCAPKTLEELEQGLMILRRLLDDLT 4CVQ Chain:A ((58-428)) ------------------------------------------------------------------------------------------------------GNKVLKLNIGNPAPFGFDAPDEILVDVIRNL-----PTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDDIRAKIT-PRTRGIVII-NPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEML-ASMRLCANVPAQHAIQTALGGYQSISEFITPG-GRLYEQRNRAWELINDIPGVSCVKPRGALYMFPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWP---WPDHFRIVTLP--RVDDIELSLSKFARFLSGY-

General information: TITO was launched using: RESULT: Template: 4CVQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1924 -230593 -119.85 -668.39 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -119.85 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_4CVQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CVQ-query.scw PDB file : Tito_Scwrl_4CVQ.pdb: