Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVDYEREEEHTTPEQPKRSKKGYFLSSLIGVIVGAVLMAFIMPYLSNEGLDTGALDQQQNNNGRESIRTVNVSVNNAVTKIVSNMSPAVVGVVNIQKSDIWGESGEAGSGSGVIYKKNDHSAYVVTNHHVIEGASQIEISLKDGSRVSADLVGSDQLMDLAVLRVKSDKIKAVADFGNSDKVKSGEPVIAIGNPLGLEFAGSVTQGVISGTERAIPVDSNGDGQPDWNAEVLQTDAAINPGNSGGALLNMDGKVIGINSMKIAESAVEGIGLSIPSKLVIPVIEDLERYGKVKRPFLGIEMKSLSDIASYHWDETLKLP----KNVTNGAVVMGVDAFSPAGKAGLKELDVITEFDGYKVNDIVDLRKRLYQKKVGDRVKVKFYRGGKEKSVDIKLSSADQLGS 1LCY Chain:A ((15-323)) ---------------------------------------------------------------------------NFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAA---DGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFAL--QNTITSGIVSSAQRP-------------NVEYIQTDAAIDFGNAGGPLVNLDGEVIGVNTMKVT----AGISFAIPSDRLREFLHR---------RYIGVMMLTLSPSIL----AELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVR---TQSQLAVQIRRGRETLTLYVTPEV------

General information: TITO was launched using: RESULT: Template: 1LCY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1680 -111544 -66.40 -396.95 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -66.40 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_1LCY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LCY-query.scw PDB file : Tito_Scwrl_1LCY.pdb: