Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMKQFEIAAIPGDGVGKEVVAAAEKVLHTAAEVHGGLSFSFTAFPWSCDYYLEHGKMMPEDGIHTL-TQFEAVFLGAVGNPKLVPDHISLWGLLLKIRRELELSINMRPAKQMAGITSPLLHPNDFDFVVIRENSEGEYSEVGGRIHRGDDEIAIQNAVFTR----------KATERVMRFAFELAKKRRSHVTSATKS------NGIYH---AMPFWDEVFQQTA-------ADYSGIETSSQHIDALAAFFVTRPETFDVIVASNLFGDILTDISSSLMGSIGIAPSANINPSGKYPSMFEPVHGSAPDIAGQGLANPIGQIWTAKLMLDHFGEEELGAKILDVMEQVTADG-IKTRDIGGQSTTAEVTDEICSRLRKL 3TY3 Chain:B ((9-363)) ---RRIVLGLIPADGIGKEVVPAARRLMENLPAKHK-LKFDFIDLDAGWGTFE---KALPERTVERLKTECNAALFGAVQSPTHKVAGYS--SPIVALRKKMGLYANVRPVKSLDGAKG-----KPVDLVIVRENTECLYVK---------EERMVQNTPGKRVAEAIRRISEEASTKIGKMAFEIAKSRQKIRESGTYSIHKKPLVTIIHKSNVMSVTDGLFRESCRHAQSLDPSYASINVDEQIVDSMVYRLFREPECFDVVVAPNLYGDILSDGAASLIGSLGLVPSANV---GDNFVMSEPVHGSAPDIAGRGIANPVATFRSVALMLEFMGHQDAAADIYTAVDKVLTEGKVLTPDLGGKSGTNEITDAVLAN----

General information: TITO was launched using: RESULT: Template: 3TY3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1811 -173837 -95.99 -536.53 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -95.99 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_3TY3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TY3-query.scw PDB file : Tito_Scwrl_3TY3.pdb: