Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRVTRLLEVKDLAISFKTYGGEVQAIRGVNFHLDKGETLAIVGESGSGKSVTSQAIMKLIPMPPGYFK--RGEILFEGKDLVPLSEKEMQNVRGKEIGMIFQDP-----MTSLNPTM----KVGKQITEVLFKHEKISKEAAK-KRAVELLELVGIPMPEKRVNQFPHEFSGGMRQRVVIAMALAANPKLLIADEPTTALDVTIQAQILELMKDLQKKIDTSIIFITHDLGVVANVADRVAVMYAGQIVETGTVDE----IFYDPRHPYTWGLLASMPTLESSGEEELTAIPGTPPDLTNPPKGDAFALRSSYAMKIDFEQEPPMFKVSDTHYVKSWLLHPDAPKVEPPEAVKAKMRKLANTFEKPVLVREVE 1G9X Chain:C ((16-249)) -------------------YFGEFKALDGVSISVCKGDVTLIIGPNGSGKST-------LINVITGFLKADEGRVYFENKDI---TNKEPAELYHYGIVRTFQTPQPLKEMTVLENLLIGEINPGESPLNSLFYKKWIPKEEEMVEKAFKILEFLKLSHLYDRK---AGELSGGQMKLVEIGRALMTNPKMIVMDEPIAGVAPGLAHDIFNHVLELKAKGITFLI-IEHRLDIVLNYIDHLYVMFNGQIIAEGRGEEEIKNVLSDPK-----------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1G9X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1030 -126865 -123.17 -581.95 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : -123.17 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_1G9X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G9X-query.scw PDB file : Tito_Scwrl_1G9X.pdb: