Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLQLKEKLVLITGSTSGIGKAAAKSFLQEGAAVIVNGRKQ-ETVDRTIEEL-SGYGT-VHGAAADLSKTDEAAAFIEKVNE-IGDIDILVNNLGFFEVKDFADVTDEEWNQYFEVNVMSAVRTSRHFLPKMLAKNSGRILNIASEAGVKPLPTMIPYSMTKTALISLSRGMAEMTKGTNVTVNSVLPGPTWTEGVASYMEGAAQAAGQDTDTFIKDYFKVNEPTSLIQRYATAEEVANTIVFLASDAASAINGTAQRVEGGIIRSL 2YZ7 Chain:C ((2-256)) ----LKGKKAVVTGSTSGIGLAMATELAKAGADVVINGFGQPEDIERERSTLESKFGVKAYYLNADLSDAQATRDFIAKAAEALGGLDILVNNAGIQHTAPIEEFPVDKWNAIIALNLSAVFHGTAAALPIMQKQGWGRIINIASAHGLVASVNKSAYVAAKHGVVGLTKVTALENAGKGITCNAICPGWVRTPLVEKQIEAISQQKGIDIEAAARELLAEKQPS---LQFVTPEQLGGAAVFLSSAAADQMTGTTLSLDGG-----

General information: TITO was launched using: RESULT: Template: 2YZ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1326 -16508 -12.45 -65.77 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : -12.45 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_2YZ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YZ7-query.scw PDB file : Tito_Scwrl_2YZ7.pdb: