Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYIMKQSGWLELICGSMFSGKSEELIRRVKRATYAKQEVRVFKPVIDNRYSEAAVVSHNGTSMTSYAISSAADIWDHISESTDVVAVDEVQFFDQEIVEVLSSLADKGYRVIAAGLDMDFRGEPFGVVPNIMAIAESVTKLQAVCSVCGSPASRTQRLIDGKPASYDDPVILVGAAESYEARCRHHHEVPGKSKK 2J9R Chain:A ((24-210)) ---INQNGWIEVICGSMFSGKSEELIRRVRRTQFAKQHAIVFKPC-----------------VKAVPVSASKDIFKHITEEMDVIAIDEVQFFDGDIVEVVQVLANRGYRVIVAGLDQDFRGLPFGQVPQLMAIAEHVTKLQAVCSACGSPASRTQRLIDGEPAAFDDPIILVGASESYEPRCRHCHAVP-----

General information: TITO was launched using: RESULT: Template: 2J9R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 866 -105907 -122.29 -622.98 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -122.29 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_2J9R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J9R-query.scw PDB file : Tito_Scwrl_2J9R.pdb: