Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQSKGKKRNPGLKIPKEAFEQPQTSSTPPRDLDSKACISIGNQNFEVKADDLEPIMELGRGAYGVVEKMRHVPSGQIMAVKRIRATVNSQEQKRLLMDLDISMRTVDCPFTVTFYGALFREG------DVWICMELMDT-SLDKFYKQVIDKGQTIPEDILGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINALGQVKMCDFGISGYLVDSVAKTIDAGCKPY-MAPERINPELNQKG-YSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSAEFVDFTSQCLKKNSKERPTYPELMQHPFFTLHESKGTDVASFVKLILGD 4U43 Chain:A ((15-290)) -----------------------------------------DLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKL--EINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNT--KGNTLKEDWIAYISREILRGLAHLHIH-HVIHRDIKGQNVLLTENAEVKLVDFGV-----------NTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDM-HPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRT

General information: TITO was launched using: RESULT: Template: 4U43.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -126803 for 2062 contacts (-61.5/contact) + 2D Compatibility (PS) -27266 + (NN) -16173 + (LL) 2584 1D Compatibility (HY) -20000 + (ID) 4550 Total energy: -192208.0 ( -93.21 by residue) QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_4U43.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U43-query.scw PDB file : Tito_Scwrl_4U43.pdb: