Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHTVTQTSLYGKDLLTLKDLSEEDINALLAEAGELKQNKI----QPIFHGKTLAMIFEKSSTRTRVSFEAGMAQLGGSALFLSQKDLQLGRGETVADTAKVLSGYVDAIMIRTFEHEKVEELAKEADIPVINGLTDKYHPCQALADLLTIKE-IKGK-LKGVKVAYIGDG-NNVAHSLMIGCAKMGCDISIASPKGYEVLDEAAEAAKTYAL--QSGSSVTLTDDPIEAVKDADVIYSDVFTSMGQEAEEQ-ERLAVFAPYQVNAALVS-HAKPDYTFLHCLPAHREE------------------EVTAEIIDGPNSAVFQQAENRLHVQKALLKAILYKGESSKNC 1DUV Chain:G ((1-333)) ------SGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLTGKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYDGIQYRGYGQEIVETLAEYASVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLVYAGDARNNMGNSMLEAAALTGLDLRLVAPQA--CWPEAALVTECRALAQQNGGNITLTEDVAKGVEGADFIYTDVWVSMGEAKEKWAERIALLREYQVNSKMMQLTGNPEVKFLHCLPAFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK-------

General information: TITO was launched using: RESULT: Template: 1DUV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1795 -161171 -89.79 -530.17 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain G : 0.82 3D Compatibility (PKB) : -89.79 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_1DUV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DUV-query.scw PDB file : Tito_Scwrl_1DUV.pdb: