Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLSFLVSLVVAIVIGLIGSAIVGNRLPGGIFGSMIAGLIGAWIGHGLLG-T--W--GPS------LAGFAIFPAIIGAAIFVFLLGLIFRGLRKEA 5AZB Chain:A ((64-118)) -------------------------------AGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSFHGGLIGVIVVMIIFA----------

General information: TITO was launched using: RESULT: Template: 5AZB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 120 -15236 -126.97 -346.27 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -126.97 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.215

(partial model without unconserved sides chains): PDB file : Tito_5AZB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AZB-query.scw PDB file : Tito_Scwrl_5AZB.pdb: